EPR on Flavoproteins.
نویسندگان
چکیده
Flavoproteins often employ radical mechanisms in their enzymatic reactions. This involves paramagnetic species, which can ideally be investigated with electron paramagnetic resonance (EPR) spectroscopy. In this chapter we focus on the example of flavin-based photoreceptors and discuss, how different EPR methods have been used to extract information about the flavin radical's electronic state, its binding pocket, electron-transfer pathways, and about the protein's tertiary and quaternary structure.
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ورودعنوان ژورنال:
- Methods in molecular biology
دوره 1146 شماره
صفحات -
تاریخ انتشار 2014